Studies on the carbohydrate metabolism in the mammary gland

II Structural types of phosphoric esters in rat mammary glands

Abstract

A paper-chromatographic study was conducted on the phosphoric ester-patterns in the extracts from slices and homogenates of mammary glands of albino rats in the virgin, lactation and post-lactation periods, respectively.
When the ethanol and trichloracetic acid extracts from the mammary glands in the virgin, initial lactation, mid-lactation and post-lactation periods, respectively, chromatographed on acid-washed paper in n-butanol-acetic acid-water mixture, and spraying with FeCl3 and with salicylsulfonic acid, 8, 10, 10 and 9 spots, respectively, of phosphoric esterswere shown to be present. Mammary glands in the four periods were shown to contain glucose-l-phos-phate, fructose-1, 6-diphosphate, glycero-phos phate, and five unknown substances. Fructose-6-phosphate was identified in the glands in the lacta-tion and post-lactation periods, whereas adenosine-triphosphate was undoubtedly present, though in smiler amounts, in lactating mammary glands. Increase of glucose- l-phosphate and disappearance of galactose were most rapid in lactating mammary glands in the incubates of gluucose and galactose.