Abstract
For the purpose of analyzing changes in protein fractions of various body fluids during development chickembryo serum was, first of all, studied paperelectrophoretically all through development, as embryonal environments have an advantage to be classified into two categories, internal and external.
Total protein of chickembryo serum was found to increased along with development of the embryo, is the case with the human fetus, though it was always smaller in quantity in the former than in the latter.
Electrophoretic diagrams were divided into three components during development and five after hatching. In the changes of percentage of the fractions, a serum fraction with the largest rate of flow (SI) showed a decrease in the middle period and an increase thenceforce. SII indicated an increase in the middle period and a decrease thereafter, and SIII a decrease after an increase at the beginning and a constancy after the middle period.
The amount of SI showed a decrease in the middle period of development and, at the end a constancy afteran abrupt increase, though samller than albumin of human fetal serum. SII increased grad ually all through the development, similarly to the changes of α1-and α2-globulin, although at the end such sudden increase as was seen in β-globulin was not observed in SII. SIII indicated a steady increaseall the while after fluctuation at the beginning, contrary to the abrupt increase of γ-globulin at the end of gestation.
