Studies on serum esterase polymorphism and attempt on their application to animal breeding

III. Differences of serum esterase zymograms among strains of rats

Abstract

Differences of serum esterase zymograms among three strains of rats were observed by starch-gel electrophoresis and histochemical methods.
The results were summarized as follows:
1) Fourteen esterase zones in rats were separated by starch-gel electrophoresis, and in these esterase zones, Es-3, Es-4, Es-5 and Es-9 zone were observed in all sera of three strains but not Es-2 and Es-6 zone.
2) Sera from the USC strain didn't show Es-1 zone, the fastest migrating zone whereas sera from the Wister and the Donryu strain revealed that.
3) In the slowest migrating region, serum cholinesterase isozymes (Es-13, Es-14 and Es-15 zone) showed variation among strains or individuals, and in this region the three phenotypes, ChE-I, ChE-II, and ChE-IV, were observed in both sexes.
4) Es-13 zone in the ChE-I type showed considerably different activities among strains and sexes. The lowest activity was found in the USC strain and the highest in the Wistar strain, and in females the activity-levels of Es-13 zone were always higher than those in males.