Studies on the toxic principle in a certain by-product of rice oil rendering

II. A survey of a toxic substance

Abstract

It was reported in the first memoir of the studies that TF (a toxic by-product) was toxic on the chick, the syndrome was closely resembled to that of chick edema disease and toxic principle appeared in the unsaponifiable fraction of TF.
In order to investigate the toxic principle, several elements in TF were analyzed first of all. The concern of contained As and Hg in toxicity was denied. However, the concentrations of Cl in TF and its unsaponifiable fraction were remarkably high and almost all Cl in the unsaponifiable fraction was concentrated on the toxic fraction (F 2) by alumina column chromatography.
From these results, the authors presumed that chlorine compound would participate in toxicity of TF.
The analysis of the same chlorinated biphenyl (sold as Kaneclor 400, KC 400) as was used for heat tranfer medium in rice oil rendering process connected with TF by using gas-liquid chromatography revealed that F 2 contained KC 400. Furthermore, most of Cl in TF was calculated to result from KC 400. Consequently it became quite possible that toxicity of TF was due to KC 400 (contained in it).