Abstract
The extract of dried Ladino Clover meal prepared with the medium of dil HCl and aceton (1: 1) at pH 1-2 were infused into the rumen of goat through fistula and its influence on the formation of vitamin B2, B6, pantothenic acid and nicotinic acid in the rumen was examined.
In the period of usual feeding, the goat was given 500 grams of dried hay at 9:00 A. M. and then 250 grams of concentrate at 4: 30 P. M. as the basal feeding. In the period of experiment, the clover extract was infused into the rumen through fistula before feeding of dried hay at 9: 00 A. M. and this infusion was executed for 14 days successively. In both of the periods rumen contents were taken through fistula before the infusion of extract or the feeding of dried hay at 9: 00 A. M. for 7 days from the eighth through fourteenth day.
The amounts of B2, B6, pantothenic acid and nicotinic acid in these rumen contents were estimated by the methods of chemical and microbiological assay and these values in both periods were compared with each other.
As the results it was ascertained that in the period of usual feeding the values of B2, B6, pantothenic acid and nicotinic acid were 4.6-7.2μg, 7.7-9.2μg, 47.0-66.0μg and 31.9-48.5μg and those values in the period of experiment were 5.6-8.2μg, 7.3-8.1μg, 49.3-103.4μg and 11.4-30.1μg Per gram of dry matter of each samples respectively.
From these results it was concluded that the infusion of clover extract into the rumen should have influenced on the activity of microorganisms in the rumen and the biosynthetic formation of vitamins was affected by the extract.
