Inhibin-like Substance in Goat Testicular Extracts and Seminal Plasma

Abstract

Inhibin-like activity in goat testis, accessory reproductive glands and seminal plasma was measured. It was not detected in the extracts of accessory reproductive glands by in vitro (mouse pituitary incubation) assay. The goat testicular extracts (GTE) and seminal plasma (GSP) showed similar inhibin activity in both in vitro and in vivo (mouse uterine weight) assays. Fractionation of GTE and GSP by ultrafiltration demonstrated that the inhibin-like substance in GTE had a molecular weight of 10, 000-30, 000 and>100, 000, while that in GSP had 5, 000-10, 000 and 10, 000-30, 000 daltons. The activity was destroyed by heating at 100°C for 5min.