1986 Volume 57 Issue 12 Pages 1000-1009
A new collection method for pancreatic juice in chickens was developed. Duodenum of the chick anesthetized with diethyl ether was cannulated with Tygon R tube, through which physiological saline was continuously infused with the aid of a peristaltic pump in order to prevent a plug problem. Pancreatic secretions were successfully collected during an experimental period of 60 min as the mixture of the saline with intestinal juice. By this method, neither the blockage problem nor the irregular secretion of the pancreatic juice was observed. A steady, relatively small basal output of enzymes, amylase, trypsinogen and chymotrypsinogen, occured when physiological saline or the end products of digestion (glucose, methionine and lysine) were administered by the wing vein injection. Cholecystokinin had an immediate effect on pancreatic enzyme secretion and this response was in a dose dependent fashion. The injection of cholecystokinin seemed to have selective stimulation favoring the secretion of chymotrypsinogen followed by amylase and trypsinogen. The injection of cholecystokinin plus lysine increased the secretion of all enzymes more than that observed with cholecystokinin alone, whereas the injection of cholecystokinin plus glucose stimulated only an amylase secretion. The regulatory mechanism of pancreatic digestive enzyme secretion is discussed.