Determination of Vitamin A in Feeds by HPLC with Anthracene as a Standard
1987 Volume 58 Issue 10 Pages 813-816
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1987 Volume 58 Issue 10 Pages 813-816
A procedure for the quantitative analysis of Vitamin A (retinol) in feed by high performance liquid chromatography (HPLC) was established.The system separated retinol in 20 min using a LiChrosorb SI-100 column with a mobile phase of n-hexane/dichloromethane/ethanol=90/9/1 at 1.5 ml/min.The detection was carried out with a fluorescence spectrophotometer set at 340 nm for excitation and at 460 nm for emission. Anthracene was found to be useful as a standard substance in this system.The minimum detectable amount was 20 μg/kg.The HPLC assay showed a recovery of 99±2% for added retinol.Retinol content has 378 μg/kg in dairy feed, 1330-2560 μg/kg in swine feed and 872-2570 μg/kg in chick feed, respectively.
