Molecular Cloning of a Bovine Minisatellite DNA Homologous to Mouse Minisatellite

Abstract

We have isolated a genomic DNA clone containing a bovine homologue of mouse minisatellite. Southern blot analysis and sequencing revealed that a 6.5Kb BamHI fragment within the clone contained repetitive DNA consisting of tandem repeats with a core sequence GGCTGTGTGT. The cloned bovine minisatellite DNA detected high restriction fragment length polymorphism (RFLP) among individuals when used as a probe, indicating that the probe could be a useful for DNA fingerprinting.