Ascorbic Acid-2-Phosphate Enhances Adipocyte Differentiation of Cultured Stromal Vascular Cells Prepared from Bovine Perirenal Adipose Tissue

Abstract

Stromal vascular cells isolated from perirenal adipose tissue of adult beef cattle were cultured, and the actions of L-ascorbic acid-2-phosphate (Asc-P) on the adipocyte differentiation of these cells were investigated. The addition of Asc-P into culture medium increased adipogenic marker enzyme (glycerol-3-phosphate dehydrogenase) activity of the cells as well as the number of lipid-laden cells countable under microscopic observation, at concentrations of 50 and 500μM. Cytosolic protein content was unaffected by the Asc-P treatments. In conclusion, Asc -P enhances adipocyte differentiation of bovine preadipocytes in vitro. The results raise the possibility that adipose tissue growth in beef or dairy cattle could be manipulated by controlling blood levels of vitamin C.