Studies on Follicular Hormones. VIII. Quantitative Analysis of Estrone and Estradiol in Pregnant Mare and Stallion Urine by Paper Chromatography, measuring the Area of Colored Spots

Abstract

Two-and-two dose assay method was applied for the quantitative analysis of estrone and estradiol by paper chromatographic analysis, measuring the area of spots, and fairly satisfactory results were obtained. In a fresh urine of a pregnant mare, an average 2% of free estrone and 6% of free estradiol, 98% of conjugate estrone, and 94% of conjugate estradiol were found by this method. About equal amounts of estrone (39 γ/cc.) and estradiol (40 γ/cc.) were determined in the stored stallion urine.