抄録
Spin-lattice relaxation times (T_1) and spin-spin relaxation times (T_2) of water in fig (Ficus carica L.) fruit with ripening were determined by inversion recovery (180°-τ-90° pulse sequence) method and CPMG method using ^1H-NMR, respectively. Spectral recovery observation showed that a spectrum in fig tissues consisted of several spectra having different chemical shifts and recovery times with ripening. Furthermore, semi-log plots of signal intensity of ^1H-NMR revealed that water in the tissues consisted of three water components with T_1 values ranging about below 0.1s (component I), between 0.6s and 1.0s (component II), and over 1.2s (component III), respectively. Furthermore, T_2 values showed long fraction ranging over 0.1s and short fraction. The highly mobile water could be estimated as free water derived from vacuoles, while water with restricted mobility was as loosely bound water and bound water from cytoplasm and the apoplastic region, respectively. In mature receptacle tissues especially, component n disappeared but component III appeared as major fraction with significant water uptake in the tissues. Disruption of the apoplastic region in receptacle tissues of mature fig fruit seems to promote increase in free water and decrease in bound water with cell enlargement. These results indicated that water compartments reflected water absorption and cellular heterogeneity in both receptacle and drupelet tissues.
