抄録
This paper dealt with the osmotic response and subsequent injury of isolated cells in suspension resulted from concentration of extracellular NaCl solution, which plays an important role in the damage mechanism during freezing. Survival of human prostate cancer cells during and after exposure to hyperosmotic NaCl solution was measured using a perfusion microscope as a function of NaCl concentration, the time of exposure and the rate of change in concentration, to examine the mechanism of cell injury. The influence of osmotic injury on the cell damage during slow freezing of cells was also examined quantitatively by comparing the perfusion experiment with a freezing experiment.
