Role of Cryoprotectants in Freeze Concentration Technology for Gene Delivery Process

抄録

There is an urgent need to develop highly effective techniques for successful and efficient gene delivery. In this study, we demonstrated the use of cryoprotectants in the newly developed freeze concentration technique in the gene delivery system. We found that at extremely low temperatures, gene transfection was effectively enhanced. Additionally, by comparing the effects of the commercially available cryoprotectant DMSO against a synthesized polyampholyte cryoprotectant, we were able to establish that the latter was more effective at increasing the rate of successful gene transfection. This particular feature could be attributed to the higher freeze concentration factor of polyampholytes in comparison with DMSO, under frozen conditions. Therefore, the study demonstrated the unique efficacy of polyampholytes as a cryoprotectant using the freeze concentration method for efficient in vitro gene delivery.