抄録
There is an urgent need to develop highly effective techniques for successful and efficient gene
delivery. In this study, we demonstrated the use of cryoprotectants in the newly developed freeze
concentration technique in the gene delivery system. We found that at extremely low temperatures,
gene transfection was effectively enhanced. Additionally, by comparing the effects of the commercially
available cryoprotectant DMSO against a synthesized polyampholyte cryoprotectant, we were able to
establish that the latter was more effective at increasing the rate of successful gene transfection. This
particular feature could be attributed to the higher freeze concentration factor of polyampholytes in
comparison with DMSO, under frozen conditions. Therefore, the study demonstrated the unique
efficacy of polyampholytes as a cryoprotectant using the freeze concentration method for efficient in
vitro gene delivery.
