抄録
The post-thaw cell survival rate as a function of cooling rate commonly has a bell shape with an optimal
cooling rate. This characteristic is understood on the basis of two-factor hypothesis, i.e. cell injuries due to
extracellular- and intracellular ice formations (EIF and IIF). The IIF-induced injury was already modeled
successfully based on intracellular ice nucleation theory. In this study, first the experiment was performed to
investigate the post-thaw cell viability and influence of cell freezing-patterns (EIF and IIF) to the cell
viability. Next, the mathematical model with reaction kinetic formulation, developed previously by the
authors, was applied to the experiment to describe the EIF-induced injury. An inverse problem analysis based
on the experiment was performed to compare the cell survival rate as a function of cooling rate between the
experiment and prediction by the model. The result shows that the EIF-induced cell injury is successfully
described by the reaction kinetic model.
