抄録
Human pluripotent stem cells are expected to be a valuable resource in the field of cell therapy and drug
discovery. Cryopreservation is a key technique to realize these applications which will require large cell
numbers. However, the common protocols for the cryopreservation including dissociation into single cells
have many steps with technical difficulties. Here, we report on an efficient method for the cryopreservation
with the cells maintained adherent on culture dishes to save work. In order to establish the method, we
employed the processes for the avoidance of extreme undercooling and determined the optimum cooling rate.
