カルボキシル基導入ポリリジンを用いた植物培養細胞の予備凍結法による超低温保存

抄録

Suspension-cultured cells of Oryza sativa and Celosia cristata were cryopreserved by the pre-freezing method. Dimethyl sulfoxide (DMSO), which is often used as a cryoprotectant, is cytotoxic. Therefore, in this study, we examined the effect of carboxylate polylysine (PLL) as a cryoprotectant in replacement of DMSO. It was found that cryopreservation in Oryza sativa with 5% ethylene glycol and 3.5% PLL resulted in significantly higher survival rates than that with 5% (v/v) DMSO and 10% (w/v) glucose and in Celosia cristata with 10% (w/v) glucose and 3.5% (w/v) PLL resulted in nearly survival rates that with 5% (v/v) DMSO and 10% (w/v) glucose. Our results indicate that PLL is effective as a cryoprotectant for cryopreservation of cultured plant cells.