Cell Structure and Function
Online ISSN : 1347-3700
Print ISSN : 0386-7196
ISSN-L : 0386-7196
REGULAR ARTICLES
Effects of Aluminum on Plasma Membrane as Revealed by Analysis of Alkaline Band Formation in Internodal Cells of Chara corallina
Mutsuko TakanoTeruo Shimmen
著者情報
キーワード: Alkaline band, Aluminum, Ca2+, Chara, Malic acid, OH-
ジャーナル フリー

1999 年 24 巻 3 号 p. 131-137

詳細
抄録
To study the mechanism of aluminum toxicity in plant cells, the effects of aluminum on alkaline band formation were analyzed in the internodal cells of Chara. After cells were treated with AlCl3, they were examined for their capacity to develop alkaline bands. Treating cells with AlCl3 medium at pH 4.5 completely inhibited alkaline band formation. When either CaCl2 or malic acid was added to the AlCl3 medium(pH 4.5), it did not produce an ameliorative effect, whereas addition of both CaCl2 and malic acid induced a significant ameliorative effect. It was found that treatment at pH 4.5 in the absence of AlCl3 strongly inhibited alkaline band formation. This inhibition by the low pH(4.5) treatment was effectively ameliorated by CaCl2. At higher pH(5.0), malic acid alone produced a significant ameliorative effect on aluminum inhibition of alkaline band formation, but CaCl2 did not. Recovery from aluminum inhibition was also studied. When cells treated with AlCl3 at pH 4.5 were incubated in artificial pond water, they could not recover the capacity to develop alkaline band. When either malic acid or CaCl2 was added to artificial pond water, cells recovered their alkaline band formation. It was concluded that one of the primary targets of aluminum is the plasma membrane and that aluminum affects the plasma membrane from the cell exterior at the beginning of the treatment(within 24 h). It was also suggested that the aluminum treatment impairs the HCO3- influx mechanism but not the OH- efflux mechanism.
著者関連情報
© 1999 by Japan Society for Cell Biology
前の記事 次の記事
feedback
Top