Insulin-Degrading Enzyme Exists Inside of Rat Liver Peroxisomes and Degrades Oxidized Proteins

Masashi Morita; Igor V Kurochkin; Kiyoto Motojima; Sataro Goto; Tatsuya Takano; Shoji Okamura; Ryuichiro Sato; Sadaki Yokota; Tsuneo Imanaka

doi:10.1247/csf.25.309

REGULAR ARTICLE

Insulin-Degrading Enzyme Exists Inside of Rat Liver Peroxisomes and Degrades Oxidized Proteins

Masashi Morita, Igor V Kurochkin, Kiyoto Motojima, Sataro Goto, Tatsuya Takano, Shoji Okamura, Ryuichiro Sato, Sadaki Yokota, Tsuneo Imanaka

Author information

Masashi Morita
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
Igor V Kurochkin
Department of Biochemistry, School of Pharmaceutical Sciences, Toho University
Kiyoto Motojima
Department of Biochemistry, School of Pharmaceutical Sciences, Toho University
Sataro Goto
Department of Biochemistry, School of Pharmaceutical Sciences, Toho University
Tatsuya Takano
Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University
Shoji Okamura
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
Ryuichiro Sato
Department of Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, The University of Tokyo
Sadaki Yokota
Biological Laboratory, Yamanashi Medical University
Tsuneo Imanaka
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

Keywords: insulin-degrading enzyme, subcellular localization, peroxisomes, proteolysis, oxidized proteins

JOURNAL FREE ACCESS

2000 Volume 25 Issue 5 Pages 309-315

DOI https://doi.org/10.1247/csf.25.309

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Abstract

Insulin-degrading enzyme (IDE) was detected by immunoblot analysis in highly purified rat liver peroxisomes. IDE in the peroxisomal fraction was resistant to proteolysis by trypsin and chymotrypsin under conditions where the peroxisomal membranes remained intact. After sonication of the peroxisomal fraction, IDE was recovered in the supernatant fraction. Further, the localization of IDE in the peroxisomes was shown by immunoelectron microscopy. In addition, IDE isolated from peroxisomes degraded insulin as well as oxidized lysozyme as a model substrate for oxidized proteins. These results suggest that IDE exists in an active form in the matrix of rat liver peroxisomes and is involved in elimination of oxidized proteins in peroxisomes.

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