Cell Structure and Function
Online ISSN : 1347-3700
Print ISSN : 0386-7196
REVIEW
Spinning-disk Confocal Microscopy — A Cutting-Edge Tool for Imaging of Membrane Traffic
Akihiko Nakano
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Volume 27 (2002) Issue 5 Pages 349-355

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Abstract

Confocal laser scanning microscopy is experiencing a revolution in speed from the world of seconds to that of milliseconds. The spinning Nipkow disk method with microlenses has made this remarkable innovation possible. In combination with the ultrahigh-sensitivity, high-speed and high-resolution camera system based on avalanche multiplication of photoconduction, we are now able to observe the extremely dynamic movement of small vesicles in living cells in real time.

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© 2002 by Japan Society for Cell Biology
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