Cell Structure and Function
Mitosis-Coupled, Microtubule-Dependent Clustering of Endosomal Vesicles around Centrosomes
Hiroyuki TakatsuYohei KatohTomoko UedaSatoshi WaguriTakashi MurayamaSenye TakahashiHye-Won ShinKazuhisa Nakayama
Author information
JOURNALS FREE ACCESS FULL-TEXT HTML

Volume 38 (2013) Issue 1 Pages 31-41

Details
Full Text-HTML Download PDF (4035K) Contact us
Abstract

Upon cell division, not only cells themselves but also their organelles undergo drastic shape changes, although the behaviors of organelles other than the Golgi apparatus remain poorly understood. We followed the spatiotemporal changes in the localization of transferrin receptor (TfnR) and other proteins. In early mitotic phases, a population of proteins cycling through the endocytic recycling compartment (ERC) exhibits a distinct spatiotemporal change from that of Golgi proteins. In prophase/prometaphase, when the cell surface-to-volume ratio is reaching its minimum, the ERC proteins are transiently assembled around the centrated centrosome in a microtubule- and dynein-dependent manner, and soon separated polewards into two clusters concomitant with separation of duplicated centrosomes. Electron microscopic analysis revealed that endosomal vesicles containing endocytosed transferrin cluster tightly around centrosomes without fusing with one another. As cytokinesis proceeds, the clusters gradually collapse, and the ERC proteins reassemble around the furrowing equatorial region. FRAP (fluorescence recovery after photobleaching) analyses of EGFP-TfnR-expressing cells revealed minimal membrane exchange between the endosomal clusters and other cellular compartments until anaphase/telophase, when membrane traffic resumes. Our observations indicate that ERC clustering around centrosomes plays a fundamental role in restricting membrane delivery to the plasma membrane during early mitotic phases, when the cell surface-to-volume ratio reaches its minimum.

Information related to the author
© 2013 by Japan Society for Cell Biology
Previous article Next article

Recently visited articles
feedback
Top