Localization of SMAP2 to the TGN and its Function in the Regulation of TGN Protein Transport

Tomo Funaki; Shunsuke Kon; Roger E. Ronn; Yuji Henmi; Yuka Kobayashi; Toshio Watanabe; Keiko Nakayama; Kenji Tanabe; Masanobu Satake

doi:10.1247/csf.10022

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Localization of SMAP2 to the TGN and its Function in the Regulation of TGN Protein Transport

Tomo Funaki, Shunsuke Kon, Roger E. Ronn, Yuji Henmi, Yuka Kobayashi, Toshio Watanabe, Keiko Nakayama, Kenji Tanabe, Masanobu Satake

著者情報

キーワード: Arf GTPase, clathrin-coated vesicles, gene targeting, immunofluorescence, membrane traffic, trans-Golgi network, vesicular stomatitis virus

ジャーナルフリー早期公開

論文ID: 10022

DOI https://doi.org/10.1247/csf.10022

この記事には本公開記事があります。

The final version of this article is now available: Vol. 36 (2011) , No. 1 p.83

詳細

抄録

SMAP2 is an Arf GTPase-activating protein that is located and functions on early endosome membranes. In the present study, the trans-Golgi network (TGN) was verified as an additional site of SMAP2 localization based on its co-localization with various TGN-marker proteins. Mutation of specific stretches of basic amino acid residues abolished the TGN-localization of SMAP2. Over-expression of wild-type SMAP2, but not of the mutated SMAP2, inhibited the transport of vesicular stomatitis virus-G protein from the TGN to the plasma membrane. In contrast, this transport was enhanced in SMAP2 (-/-) cells characterized by increased levels of the activated form of Arf. SMAP2 therefore belongs to an ArfGAP subtype that resides on the TGN and functions as a negative regulator of vesicle budding from the organelle.

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