Abstract
Existing replica methods designed for adherent somatic cells are not applicable to non-adherent cells of tumor origin. We have developed a new, efficient replica method for the negative selection of mutants of non-adherent mouse FM3A cells. Mutagenized cells are grown clonally on soft-agar plates then allowedto proliferate upwards through layered polyester disks, thus producing replicas with the same colony patterns. Isolation of mutants with increased requirements for myo-inositol by this method is described.
