Ganglioside GM1 Localization in Cholinergic Neurons Visualized by Use of a Double Avidin-Biotin Complex System

Hiroaki Asou; Noriko Mutou; Shusuke Hirano; Tamaki Takagi; Kagemasa Kajiwara; Junichi Komatsubara; Eiichi Sugaya

doi:10.1247/csf.11.175

Hiroaki Asou, Noriko Mutou, Shusuke Hirano, Tamaki Takagi, Kagemasa Kajiwara, Junichi Komatsubara, Eiichi Sugaya

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JOURNAL FREE ACCESS

1986 Volume 11 Issue 2 Pages 175-180

DOI https://doi.org/10.1247/csf.11.175

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Abstract

Localization of ganglioside GM₁ in cholinergic neurons from the septal area of a primary culture newborn rat brain was studied with a double avidin-biotin complex system. Cholinergic neurons were identified by double immunolabeling techniques that use choline acetyltransferase (ChAT) and neurofilament (NF) protein-antibodies. ChAT-positive neurons also were stained for ganglioside GM₁ by using an avidin-biotin complex technique.

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