Constitutive Long-Term Production and Characterization of Recombinant Human Interferon-Gammas from Two Different Mammalian Cells

Abstract

Two expression plasmids (pSVIFNγ/BPV97 and pSVIFNγ/ AdDHFR) for constitutive production of human interferon-gamma (HuIFN-γ) were constructed and introduced into the two different mammalian cell lines, mouse C127 cells and Chinese hamster ovary (CHO) cells. Genetically engineered C127 and CHO cells grew on microcarriers having high productivity of HuIFN-γs for at least six months. Isoelectric focusing patterns and molecular weight analyses suggest that C127-and CHO-HuIFN-γs are glycopro-teins and that both HuIFN-γs have different molecular structures. The develop-ment of a microcarrier culture system for genetically engineered mammalian cells has enabled us to prepare glycosylated HuIFN-γs on a large scale.