The Amino Acid Sequence, Immunofluorescence and Microinjection Studies on the 15 kDa Calcium-Binding Protein from Sea Urchin Egg

Abstract

The 15 kDa protein is the most abundant low molecular weight Ca²⁺-binding protein, different from calmodulin, in eggs of sea urchin, Hemicentrotus pulcherrimus (9). The data from the amino acid sequence demonstrated that the 15 kDa protein belonged to the troponin C superfamily. Based on immunofluorescent and immunomicroscopic observations, we showed that the 15 kDa protein localized in the nuclei of fertilized eggs and mitotic apparatus of dividing eggs. Microinjection of the antibody against 15 kDa protein into sea urchin blastomeres resulted in the arresting of cell division. These results suggest that the 15 kDa protein plays an important role in mitosis of sea urchin egg.