Abstract
Human recombinant interferon, (rIFN)-γ, induced a human neuroblastoma cell line GOTO to differentiatte, but neither rIFN-αA nor -β did. To elucidate the mechanism of this rIFN-γ-specific differentiation-inducing effect, we established two rIFN-γ-resistant variant GOTO clones. They were insensitive to the growth-inhibitory and differentiation-inducing effect of 1 × 103 IU/ml rIFN-γ. They were slightly sensitive to the growth-inhibitory effect of rIFN-γ (2 × 104 IU/m1). Parental GOTO cells were very insensitive to the antivesicular stomatitis virus (VSV) effect of all three types of rIFNs and even 2 × 104 IU/ml rIFN-γ could not inhibit the cytopathic effect of 5 TCID50 VSV by 50%. The degree of this insensitivity was the same in the variant GOTO cells as in the parental GOTO cells.
