Abstract
To investigate the impaired cell surface expression of humanmajor histocompatibility antigen (HLA) in transfected L cells, we examined their intracellular localization by immunocytochemistry. HLAclass II molecules produced in transfected L cells were mainly detected in the intracellular vesicles and in the nuclear envelope as granular precipitates. The results suggest that the intracellular transport of the newly synthesized molecules in transfected L cells is impaired at some point along the pathway from the rough endoplasmic reticulum (RER) to the medial-, trans-Golgi apparatus.
