J-STAGE Home  >  Publications - Top  > Bibliographic Information

Cell Structure and Function
Vol. 17 (1992) No. 1 P 77-86

Language:

http://doi.org/10.1247/csf.17.77


We earlier discovered a novel 40-kDa protein (hsp40) induced by heat shock and other stresses in mammalianand avian cells. In this report, we purified the hsp40 in HeLa cells, using modified two-dimensional gel electrophoresis, and determined the amino terminal amino acid sequence of this protein. The hsp40 is homologous to DnaJ, an Escherichia coli heat-shock protein, as well as to DnaJ-homologous proteins in yeast such as SCJ1, Sec63/Np11, YDJ1 and SIS1. Indirect immunofluorescence staining using an anti-hsp40 polyclonal antibody demonstrated that hsp40 was localized faintly throughout the cell in non-heat-shocked cells and was accumulated in nuclei and nucleoli in heat-shocked cells. The intracellular localization of hsp40 was very similar to that of hsp70, suggesting that these two hsps colocalize in heat-shocked HeLa cells.

Copyright © Japan Society for Cell Biology

Article Tools

Share this Article