Cell Structure and Function
Online ISSN : 1347-3700
Print ISSN : 0386-7196
ISSN-L : 0386-7196
Isolation and Characterization of Nuclei from Rice Embryos
Junji YamaguchiPoh-Yam LimKazumi ArataniTakashi Akazawa
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JOURNAL FREE ACCESS

1992 Volume 17 Issue 2 Pages 87-92

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Abstract
A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated nuclei was confirmed by light microscopy as well as electron microscopy. The protein profiles of both whole nuclei and nuclear extracts obtained by SDS-PAGE, organellar marker enzyme activities, DNA and RNA analyses, and in vitro RNA synthesis, all indicate that the highly purified nuclei are isolated from rice embryos.
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© Japan Society for Cell Biology
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