Cell Structure and Function
Online ISSN : 1347-3700
Print ISSN : 0386-7196
ISSN-L : 0386-7196
A Method for the Simultaneous Demonstration of Mitochondria and Their Nucleoids in the Myxomycete Physarum polycephalum by Light Microscopy
Tsuneyoshi KuroiwaShigeyuki Kawano
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ジャーナル フリー

1977 年 2 巻 1 号 p. 55-58

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抄録
A HCl-thionine staining technique was devised for the simulta-neous demonstration of in situ mitochondria and their nucleoid in the plasmo-dium of Physarum polycephalum, as well as for isolated mitochondria from microplasmodia of the same organism. Light microscopy of samples stained by the method revealed that most mitochondria contained a particle-or rod-shaped nucleoid but an appreciable number of isolated mitochondria contained V-shaped mitochondrial nucleoids. Since such mitochondria containing V-shaped nucleoid were larger in size, it appears that these mitochondria may be dividing mitochondria.
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© Japan Society for Cell Biology
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