抄録
The effect of vitamin E on the life span of human diploid cells was investigated. Ninety percent of vitamin E solubilized into growth medium was recovered from serum lipoprotein fractions in medium after removal of micelleous forms of vitamin E by Millipore filtration. Vitamin E in growth medium was found to be easily decomposed when the medium was placed in a dish in a CO2-incubator but not in a culture bottle with a tight stopper. About 2_??_4% of vitamin E initially added to growth medium was incorporated into cells. The effect of vitamin E was investigated with parameters, such as cell attachment, cell proliferation rate and colony formation. Furthermore, in contrast to the findings by Packer and Smith, vitamin E had no effect on extending the life span of human fetal lung fibroblasts. Parallel cultures grown in the presence of 5 μg/ml hydrocortisone exhibited significant extensions in cell life span.
