Abstract
Neurofilaments consisting almost exclusively of triplet polypeptides (with molecular weights of 200, 000, 160, 000 and 68, 000) were isolated from the rat peripheral nerve. A portion of the triplet polypeptides seems to exist in a non-polymeric form that is not sedimentable by high-speed centrifugation, and is undetectable by electron microscopy. In the presence of both neurofilaments and vinblastine sulphate, a white precipitate developed at 37°C, that rapidly redissolved at 4°C. Electron microscopy showed that the appearance of the neurofilaments did not alter during their interaction with vinblastine.
