Abstract
The Chang human conjunctival cell line was used, and a mixture of cells with various numbers of nuclei was obtained by cell fusion with Sendai virus (HVJ).
Synthesis of the RNA molecule was studied by radioautography. Statistical analysis revealed that the number of cytoplasmic grains, the number of nuclear grains, and the number of grains in all the nuclei and in the cytoplasm were all linearly related to the number of nuclei in the multinuclear cells. This relationship was found to be proportionately less than the hypothetical relationship of grain count to nuclear number in mononuclear cells with the same total number of nuclei.
Cytoplasmic transfer rates were similar in mononuclear cells and in multinuclear cells that had between 2 to 19 nuclei. The transfer rates averaged 20.54±0.46% after 1 h of incubation with [3H] 5-uridine.
