Abstract
Some properties of A31 cells (a clonal line of BALB/3T3 cells) transformed by fragments of DNA of bovine adenovirus type 3 (BAV3), i.e., EcoRI D fragments (3.6 to 19.7 map units of viral genome) or HindIII J-E ligated fragments (0 to 11.9 map units) produced by restriction endonuclease digestion, were examined. These transformed cells were found to commonly contain most, if not all, of the nucleotide sequences in the region of from 3.6 to 11.9 map units of BAV3 genome, by DNA-DNA reassociation kinetics analysis. Moreover, the synthesis of virus-specific RNA was detected by DNA-RNA hybridization experiments. TrD and TrJE cells (A31 cells transformed with Eco RI D fragments and HindIII J-E ligated fragments of BAV3 DNA, respectively) as well as A31 cells transformed with whole viral DNA or BAV3, showed a rapid uptake of 2-deoxyglucose, high cell agglutinability by plant lectins and high plasminogen acivator activity, in comparison with A31 cells. All these transformed cells produced tumors when inoculated subcutaneously into adult BALB/c mice. These results indicated that all these characteristics examined are directly or indirectly controlled by the gene(s) located between the map units of 3.6 and 11.9 of the BAV3 genome.
