抄録
A method for the mass isolation of polytene nuclei of T. akamushi is described. Isolated nuclei were studied with light and electron microscopy, SDS-gel electrophoresis and DNA fragment analysis after micrococcal nuclease digestion. Histones were the major components of the chromosomal proteins. A number of non-histone proteins with a wide range of molecular weights were present, but their quantities were relatively low. The sizes of the DNA fragments cleaved by micrococcal nuclease suggested the presence of nucleosomal structures in the polytene chromosomes. The size of the DNA repeat was about 200 base pairs.
