Cell Structure and Function
Online ISSN : 1347-3700
Print ISSN : 0386-7196
ISSN-L : 0386-7196
Changes in Actin-Related Gelation of Crude Cell Extracts during Differentiation of Myeloid Leukemia Cells
Kazuhiro NagataJunji SagaraYasuo Ichikawa
著者情報
ジャーナル フリー

1983 年 8 巻 2 号 p. 171-183

詳細
抄録
Gelation of extracts of a myeloid leukemia cell line (M1) was compared before and after differentiation induced with conditioned medium (CM) from rat embryo cells. Although an extract of Mml cells, a macrophage line derived from Ml line, gelled when warmed in the presence of 2 mM MgCl2, undifferentiated M1 cells gelled only after dialysis and a supplement of exogenous actin. After differentiation had been induced, an addition of exogenous actin, but not dialysis, was needed for gelation.
Small amounts of KCl always inhibited the gelation of the control M1 cell extracts, but they promoted gelation of the CM-treated M1 and Mml cell extracts. Thus, the dialysis required for gelation of the control M1 cell extract appears to be necessary for the exclusion of endogenous KCl. Several possible mechanisms for the KCl control of gelation, as well as different requirements of exogenous actin needed for gelation are discussed based on the results of our experiments.
著者関連情報
© Japan Society for Cell Biology
前の記事 次の記事
feedback
Top