Abstract
Rat SV-3Y1 cells were stained with double immunofluores-cence. Treatment of cells with detergent and salt removed about 80 % of the antigens and revealed immunofluorescent flecks of the SV40 large T antigen and p53 bound to the nuclear skeleton. These flecks exactly corresponded to the fluorescent spots produced by antibodies against microtubule associated protein-1. The MAP-1 analogues may function in the initiation of DNA synthesis through the interaction with T-antigen and p53.
