日常検査におけるFCM測定の問題点

－gating ー

抄録

Flow cytometric analysis is indespensable for identifying immunophenotypically hematopoietic cells. In such analysis, it is the most important issue how to choose flow-cytometrically target cells, namely gating. To differentiate target cells from the other cells, three gating approaches are generally known; morphological gating derived from the SSC/FSC cytogram, specific gating of the Leuko-gate(BD) using CD45 and CD14 measurement, and two-color gating using two CD antibodies relevant to target cells. In this paper, we reviewed our experiences of the problematic issues and relevance of gating in flow cytometric analysis as a routine hematological test.