Fractionation of guinea pig leukocyte by flow cytometry using a novel MIL4/SSC parameter

抄録

The guinea pig has been one of the valuable animal models in studying various diseases and developing counter measures. However, many immunological parameters remain unclarified. Using commercially available anti-guinea pig antibodies or usual gating, leukocyte fractionation has been difficult to differentiate by flow cytometry. We found that cross-reactive anti-porcine antibody MIL4 is effective in differentiating leukocytes into 4 distinct populations by gating with SSC. Each of the 4 populations were sorted and identified in which: fraction 1 that was MIL4^negativeSSC^low was ‘lymphocyte’; fraction 2 that was MIL4^highSSC^high was ‘neutrophil’; fraction 3 that was MIL4^intermidiateSSC^intermediate was 'monocyte'; and fraction 4 that was MIL4^negativeSSC^high possessed a large PAS+ inclusion body and was CD4^high, FcR⁺, CD8^low and T-cell markers. Thus, a novel MIL4/SSC parameter enabled the differentiation of peripheral blood leukocytes of guinea pigs into 4 characteristic leukocyte fractions. This may provide a tool to define the phenotypes of each leukocyte fraction of the guinea pig.