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Online ISSN : 2424-0664
Print ISSN : 0916-6920
ISSN-L : 2424-0664
総説
フローサイトメトリ-を用いた細胞周期解析Ⅱ
―BrdU 連続ラベル法と細胞周期時間―
藤川 孝三郎宮越 稔
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2006 年 16 巻 2 号 p. 37-41

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The BrdU continuous labeling method was presented for a method that can determine the cell cycle time. In this method, the relationships are derived using an equation that n( t,τ) =αN0exp(αt) exp (ατ), where n (t,τ) is cell density of a phase τ at time t, N0 is the initial cell number at time t = 0, Tc is cell-cycle time and α = ln2 / Tc. BrdU is incorporated into DNA strand instead of TdR. Anti BrdU monoclonal antibody is used to distinguish labeled and unlabeled cells in a population and to determine the fraction. The plotting of labeled or unlabeled cell fraction against time reveals the cell cycle time.

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