2014 年 24 巻 2 号 p. 33-39
We review here flow cytometric gating methods, which were newly established and named “HAS-Flow,” to analyze ATL (adult T-cell leukemia/lymphoma) cells. Using CD7 vs. CD3 or CADM1 plots of CD4-positive lymphocytes, HTLV1- infected ATL cells can be specifically separated from other normal T-cells. These plots are different among subtypes of ATL, and reflect the disease progression from HTLV-1 infection through indolent ATL to aggressive ATL. Moreover, the change of the plots after chemotherapy could distinguish chemo-sensitive cases and predict prognosis. We are now advancing their clinical applications. For example, recurrence of ATL and engraftment after HSCT (hematopoietic stem cell transplantation) can be monitored simultaneously by combining HAS-Flow with HLA-Flow, which can analyze mixed chimerism among various cell populations after HSCT.