Abstract
Nanomedicines with a particle size larger than 50 nm cannot access pancreatic cancer cells because the fibrous stroma covering the cancer cells acts as a barrier. This means that semi-flexible polymeric DNA with a persistence length of 50 nm cannot be delivered to pancreatic cancer cells. On the contrary, it means that DNA can be delivered if the particle size is less than 50 nm. In this paper, after describing the basic design guidelines for gene vectors made of polymers, we will discuss how DNA can be folded below the persistent length and minimized to pass through the stroma. This overcomes the “no” of undeliverability and makes it possible to express genes in pancreatic cancer cells.
