抄録
Novel assay method for antisense effect of oligodeoxynucleotides was designed. Antisense oligodeoxynucleotides (21mer) complementary to the region of the translation start site of c-jun and c-fos protooncogene inhibit apoptotic FM3A cell death induced by 5-fluoro-2'-deoxyuridine. Since antisense effect can be detected as survival of the cells, non-specific cytotoxicity of oligodeoxynucleotides can de neglected in this system. Though this inhibition effect is not directly related to a certain clinical target, the system is useful for the evaluation of novel designs in oligodeoxynucleotide molecules and/or antisense carriers. Phosphorothioate and 3'-terminal modified oligodeoxynucleotides were evaluated for their antisense effect in this system.
