抄録
Peritoneal recurrence is one of the most important disease after surgery of the gastrointestinal cancer. We have reported that dextran surfate prevents malignant cells from adhering mesothelium and attenuates peritoneal metastases when malignant cells are seeded into abdominal cavity. In this report, we examined acute toxicity of dextran sulfate in mice (BDF 1, strain, male). The mice, bred in specific pathogen free, were divided into 11 groups. One was the group of mice that was given nothing, another was the group of mice that was injected physiological saline, the rest 9 groups were the groups of mice that were received dextran sulfate in abdominal cavity. Dextran sulfate was dissolved at a range of 2-8 mg/ml in saline solution in 9 stages, and was injected into abdominal cavity of the mice. Survival rate and general conditions were observed during 14 days after injection. The number of dead mice increased as the dose of dextran sulfate become large. The death was seen within 3 days after injection. Body weight turned into increasing phase and the toxic symptoms were improved 4 days later. The LD50 value of dextran sulfate, calculated following the Probit method, was 0.341 mg/g, which dose is much greater than the dose effectively prevents cancer cells from adhering.
