Abstract
Multidrug resistant (MDR) cell line, AH66DR, derived from rat ascites hepatoma AH66 parental cell, AH66P, was established and overcoming effect of conjugate of bovine serum albumin (BSA) with doxorubicin (DXR) on the cell line was examined in vitro and in vivo. The conjugate (BSA : DXR molar ratio =1 : 3.28) was accumulated in the cytoplasm of the AH66DR by reduction of efflux of the drug. The conjugate expressed the excellent antitumor activity against AH66DR (IC50=50nM) and this value was almost similar cytotoxic titer of DXR against AH66P. The conjugate also showed the most efficacious life-prolonging effect against the rats bearing AH66P as well as AH66DR cell line.
