Glutathione (GSH) is a tri-peptide that plays an important role in protecting cells and tissues against oxidative stress. So far a lot of analytical methods of glutathione have been reported. This brief review presents an overview of the analysis of glutathione in biological samples by high-performance liquid chromatography (HPLC) in recent five years, focusing on the sample pretreatment, derivatization and mass spectrometric detection.
