CHROMOSOMAL RADIOSENSITIVITY IN LYMPHOCYTES OF RABBIT INDUCED BY 60Co-GAMMA RAYS IRRADIATION IN VITRO
1971 Volume 11 Issue 2 Pages 66-78
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1971 Volume 11 Issue 2 Pages 66-78
Many investigations have been reported for the radiation induced chromosome aberration in circulating lymphocytes of man who recieved the exposure of the various kinds of ionizing radiations through medical use or accidental exposure. For the quantitative study of these chromosome aberrations, a preliminary experiment was carried out, in which the lymphocytes of rabbit was chosen as experimental material and slight modification of human peripheral blood culture technique by Moorhead et al. was introduced. In this report, the dose response relationship of radiation induced chromosome abberations of circulating lympho-cytes of rabbit was studied and the effect of anesthesia, on this relationship was investigated because it was necessary to anesthetize the rabbit in this study for obtaining of enough amount of blood. The results of the present experiments were as follows: 1) the effect of anesthesia on the chromosomal radiosensitivity was negligible, 2) chromosomal dose response relationships of blood lymphocytes of rabbit in vitro was expresed as
Y=0.6×10-3+1.11×10-3D0.18+9.14×10-5D1.5
((Y: incidence of chromosome aberations, D: radiation dose (rads)))
