1986 年 1 巻 4 号 p. 397-403
Microanalyses of bestatin and p-hydroxybestatin, possible therapeutic drugs for muscular dystrophy, in mouse muscle and serum have been accomplished by high-performance liquid chromatography with fluorescence detection. The drugs are extracted into dilute acetic acid for bestatin and perchloric acid for p-hydroxybestatin. The extracts are converted to fluorescent derivatives by means of the previously reported techniques, and the derivatives are separated on a reversed-phase column (TSK gel ODS-120T) with isocratic elution, respectively. The methods permit the quantification of bestatin and p-hydroxybestatin at concentrations of as low as 900 ng and 130 ng per g in mouse muscle, and 200 ng and 30 ng per ml in mouse serum, respectively. It was found that, in oral administration in the same doses (50 mg/kg each), bestatin is distributed at higher concentrations to the muscle than p-hydroxybestatin, though the concentrations of the drugs are almost equal in the serum.
