1990 年 61 巻 6 号 p. 592-597
In the separation process of leaf protein concentrates (LPC) from alfalfa plant, deproteinized juice of alfalfa (A-BJ) is issued as a by-product, and it contains large amounts of sugars, amino acids, and minerals. The purpose of this investigation is to examine the application effect of A-BJ application on the growth and nitrogen nutrition of alfalfa plant. The results were as follows: 1) The growth of alfalfa in vermiculite pot was stimulated by the application of A-BJ and its dilute solutions. Nodulation, nitrogen fixation, and plant total nitrogen were also increased. This stimulation was observed to be higher in under-ground parts than in above-ground parts. 2) Similar phenomena were observed on the alfalfa plant grown in soil pot condition. Moreover, the application of rhizobial suspension cultured in A-BJ medium and its supernatant resulted in the stimulation of plant growth, nodulation, and plant total nitrogen of alfalfa grown in soil culture condition. Such stimulation of plant growth was probably due to the improvement of the plant nitrogen nutrition on the process of nitrogen fixation. 3) It was considered from this investigation that the deproteinized alfalfa juice, a by-poduct of leaf protein concentrates, was a useful material for the cultivation of a nodulating crop such as alfalfa.